DNA Polymerase: functions to polymerize newly forming strands of complementary DNA in the 5 to 3 prime direction, using an existing strand of DNA as a template; may also function to proofread for errors in the new DNA strand
This foldable allows students to show the complementary pairing for both DNA replication and RNA transcription. The center shows a chart comparing and contrasting DNA and RNA. It also has students list the 3 types of RNA and write the location of both transcription and replication.
Transcription proceeds in the following general steps: One or more sigma factor protein binds to the RNA polymerase holoenzyme, allowing it to bind to promoter DNA. RNA polymerase creates a transcription bubble, which separates the two strands of the DNA helix. This is done by breaking the hydrogen bonds between complementary DNA nucleotides. RNA polymerase adds matching RNA nucleotides to the complementary nucleotides of one DNA strand.
Make genetics fun! In this dry lab students will complete a “normal” karyotype as well as an "abnormal" karyotype. Upon completing the karyotype and studying the chromosomes they will determine gender and diagnose a genetic disorder.
Real-time (RT) reverse-transcription polymerase chain reaction (PCR) is currently the most sensitive and reliable technique for detecting viruses. The technology involves making complementary DNA copies of viral RNA, multiplying the number of DNA copies and staining them with a fluorescent dye. The subtle differences in fluorescence intensity reflect the presence and quantity of viral RNA. Chapter 96
Central Dogma of Molecular Biology -DNA makes RNA -RNA makes Proteins -Proteins help DNA (and sometimes RNA) to replicate itself -And some Proteins, like those from retrovirus HIV can turn RNA back into DNA (called complementary DNA or cDNA for short)